The virus causes serious losses of yield in mung bean and blackgram. Seedborne nature of a begomovirus, mung bean yellow. Mung bean yellow mosaic disease caused by mungbean yellow mosaic begomo virus mymv is the most destructive viral disease of mungbean and mash bean not only in pakistan but also in india, bangladesh, srilanka and adjacent area of southeast asia bakar, 1981. Evaluation of mung bean genotypes against mung bean. Hepper caused by mung bean yellow mosaic gemini virus and transmitted by whitefly bemisia tabaci genn. Dull seeded, high yielding, resistant to bruchids and mungbean yellow mosaic virus vigna strain and mungbean yellow mosaic india virus. Mungbean yellow mosaic disease and its management j.
Pdf mungbean yellow mosaic virus mymv disease is one of the most vicious diseases of green gram and has been renowned in india for. Six crosses between resistant and susceptible genotypes were attempted. Pdf management of yellow mosaic disease of mungbean. Wilczek were screened for yellow vein mosaic virus yvmv disease in pre and postkharif seasons under field condition. Introduction among the three, the viruses are the most important group. The whiteflyborne mung bean yellow mosaic virus my m v in thailand was transmitted by mechanical inoculation.
Nov 22, 2019 mechanical transmission, purification and some properties of whiteflyborne mung bean yellow mosaic virus in thailand. Pcna interacts with indian mung bean yellow mosaic virus rep. Management of mungbean yellow mosaic virus mymv in. Pdf first report of mungbean yellow mosaic india virus. Bean mosaic information how to treat mosaic symptoms of. In mung bean the resistance to yellow mosaic virus and powdery mildew were introduced by. Of the various viral diseases inflicting legume crops, mungbean yellow mosaic disease is one of the most destructive and widely distributed. Management of mungbean yellow mosaic virus mymv in mungbean vigna radiata l. The effect of priming seed for 8 h with water before sowing on the incidence and severity of infection of mungbean vigna radiata l. It has potential to inflict 100% damage to this crop nene, 1972. Development and introduction of resistant cultivars are considered as the most economical and ecofriendly option to manage ymd, for which availability of stable sources of resistance is a pre.
Mung bean is attacked by several viral diseases but the most devastating is mung bean yellow mosaic virus mymv and its vector bemisia tabaci genn. Diseases and insect pests of mungbean and blackgram. First report of mungbean yellow mosaic india virus on mungbean in pakistan. Identification of stable sources of resistance to mungbean. Mungbean yellow mosaic virus begomovirus is a major exotic disease of mungbeans causing leaf discoloration and yield losses. Pdf mungbean yellow mosaic disease and its management. The yellow discoloration of pods and seeds of infected plants and symptom emergence in the very first trifoliate leaf of the plants in the field were suggestive that the virus may be seed borne, which was investigated in the present study. The disease was reported from india in 1955 on mungbean nariani, 1960. The control of mymv is often based on reducing the vector population through application of insecticides, which are ineffective under severe whitefly infestations malathi. Comparative studies between amino acid sequences of potential gene products of mung bean yellow mosaic virus and other whitefly transmitted geminiviruses. Regional agricultural research station, bangladesh agricultural research institute, rahmatpur, barisal8211. Jayappa, hk ramappa and bd devamani abstract experiments were conducted on integrated management of mymv by using different combination of insecticides and neem based pesticides during january and march in 2016. Efforts to engineer resistance against the genus begomovirus are focused mainly on silencing of complementarysense virus genes involved in virus replication. Yellow mosaic virus myv is a serious disease of pulse crops including mungbean, blackgram, frenchbean, pigeonpea and soybean.
Shiny and bold seeded, early maturing and high yielding, resistant to mungbean yellow mosaic virus vigna strain and urdbean strain and mungbean yellow mosaic india virus. Identification of yeast factors involved in the replication. Mung bean yellow mosaic india virus mymiv is a member of genus begomoviridae and its genome comprises of bipartite two components, namely dnaa and dnab, singlestranded, circular dna of about 2. Screening of mutant population m2 for mungbean yellow.
Inheritance of resistance to mungbean yellow mosaic virus. Indian mung bean yellow mosaic virus imymv, a species of the begomovirus genus in the geminiviridae family, poses a tremendous threat to legume cultivation, especially in the northern parts of india. Yellow mosaic virus disease of black gram vignamungo linn. It is incited by mungbean yellow mosaic virus mymv, which is a.
Yellow mosaic diseases are one of the most important viral diseases in mungbean. Onfarm seed priming reduces yield losses of mungbean vigna. Cellophane noodles become soft and slippery when they are soaked in hot water. Genetic analysis of resistance to mungbean yellow mosaic. Mungbeans and black gram as well as other legumes such as pigeon pea. Among four viruses presumed to be mung bean yellow mosaic virus, from india, west pakistan, sri lanka and thailand, virus purification honda et al. Mechanical transmission, purification, and some properties of whiteflyborne mung bean yellow mosaic virus in thailand. Yellow mosaic disease ymd caused by mungbean yellow mosaic virus mymv is one of the most destructive biotic production constraints in mungbean. Evaluation of mung bean germplasm for resistance against.
Understanding the inheritance of mungbean yellow mosaic virus. Govindan department of plant molecular biology and biotechnology, centre for plant molecular biology, tamil nadu agricultural university, coimbatore 641 003, india. Pronounced yellow mottling and discoloration of leaves stunting of plants covering 50. Pdf the prime aim of the study was to manage of yellow mosaic disease of mungbean against mungbean yellow mosaic virus mymv by. Mungbean, vigna radiata, third in the series of important pulse crops, still suffers from yield loss due to mungbean yellow mosaic disease. Growth and yield parameters the effect of mungbean yellow mosaic virus disease on various growth and yield parameters viz. A vein yellowing in mung bean, b yellow mosaic in mung bean, c yellow mosaic in soybean, and d severe downward curling in top crop bean. Effect of dates of sowing on incidence and severity of mungbean yellow mosaic virus and cercospora leaf spot of mungbean. Bean mosaic information how to treat mosaic symptoms of beans. It is used as an ingredient in both savory and sweet dishes. Engineering resistance against mungbean yellow mosaic india. Peas are reported to be infected with at least 30 viruses worldwide. It is widely grown in tropical and subtropical re gions as a monoculture and as a component in. The objectives of this study were to determine the inheritance of mymv resistance and to identify the role of each resistance gene.
During rolling circle replication rcr of the dna, the stability of the genome and maintenance of the stemloop structure of the replication origin is crucial. Fact sheet rains arm iosecurity rogram ramakrishnan nair, avrdc, india infected plants ramakrishnan nair, avrdc, india close up of infected leaf. Seedborne nature of a begomovirus, mung bean yellow mosaic. Yellow mosaic this disease is caused by virus, starting as small yellow specks along the veinlets and spreading over the lamina.
The germplasm was categorized in to resistant and susceptible depending upon severity of disease. No insecticide was sprayed in order to maintain the natural whitefly population. Mung bean suffers from several virus diseases but they are not well described, except for mung bean yellow mosaic virus mymv, which is widespread in south asia. The mung bean is mainly cultivated in east asia, southeast asia and the indian subcontinent. Transmission efficiency of mung bean yellow mosaic virus mymv by bemisia tabaci in mungbean. Highest vector population was recorded in control no spray with whiteflies of 6. Pdf management of yellow mosaic disease of mungbean against.
Pant v, gupta d, choudhury nr, malathi vg, varma a, mukherjee sk 2001 molecular characterization of the rep protein of the blackgram isolate of indian mung bean yellow mosaic virus. Mung bean starch, which is extracted from ground mung beans, is used to make transparent cellophane noodles also known as bean thread noodles, bean threads, glass noodles, fensi, tung hoon, mi. Nov 26, 2014 2014 mungbean yellow mosaic virus mymv. Screening of blackgram genotypes against mung bean yellow. The yellow mosaic viruses ymv infecting legumes are considered to be the most devastating begomoviruses as they incite considerable yield loss. Engineering resistance against mungbean yellow mosaic. Screening of blackgram genotypes against mung bean yellow mosaic virus disease pavishna m, kannan r, arumugam pillai m and rajinimala n abstract mung bean yellow mosaic is a major destructive disease affecting blackgram productivity in india.
The objective of this research was to study the mode of inheritance of resistance to mungbean yellow mosaic virus mymv in inter tnau red. Research activities on mung bean have been mainly focused on the development of high yielding varieties with wider adaptability, resistant to diseases like mung bean yellow mosaic virus mymv and cercospora leaf spot. Molecular studies on transmission of mung bean yellow mosaic. The most important viruses which infect this crop in the mediterranean region are bean leafroll virus blrv, bean yellow mosaic virus bymv, pea seedborne mosaic virus psbmv, and pea enation mosaic virus pemv1 and 2. Primed seeds germinated and emerged faster and more completely, resulting in the establishment of 45% more plants per unit area than nonprimed seeds. Among the biotic factors, mungbean yellow mosaic virus mymv is one of the most destructive and devastating diseases that limits the mungbean production throughout asia, including india. Onfarm seed priming reduces yield losses of mungbean. Mung bean yellow mosaic virus descriptions of plant viruses. Singh, inheritance of resistance to mungbean yellow mosaic virus in greengram, anajps inheritance of resistance to mungbean yellow mosaic virus mymv in inter and intra specific crosses of mungbean vigna radiata nals of agricultural research, vol. The whiteflyborne mungbean yellow mosaic virus mymv in thailand was transmitted by mechanical inoculation. Like other members of the potyvirus genus, it is a monopartite strand of positivesense, singlestranded rna surrounded by a capsid made for a single viral encoded protein. Among the several buffers used in attempted transmissions, 0. Mechanical transmission, purification and some properties of whiteflyborne mung bean yellow mosaic virus in thailand. Molecular studies on transmission of mung bean yellow.
Bean yellow mosaic virus an overview sciencedirect topics. The crop is attacked by mung bean yellow mosaic virus mymv, powdery mildew, cercospora leaf spot, sclerotium blight, leaf blight, scab and charcoal rot. Sedimentation profiles of purified extracts from mung bean yellow mosaic infected top. Of the various viral diseases inflicting legume crops, mungbean. The mungbean yellow mosaic virus disease incidence was recorded for all the plants in m15 generation of the selected 250 mutants. Mode of inheritance of resistance to mungbean yellow mosaic virus mymv in mungbean vigna radiata l. The present investigation was to identify resistant blackgram. A majority of the begomoviruses have singlestranded bipartite genomes, and the begomoviruses are the most abundant species of the geminiviruses. The symptoms of bean yellow mosaic bymv vary again, depending upon the virus strain, stage of growth at the time of infection and variety of bean. Molecular studies on transmission of mung bean yellow mosaic virus mymv by bemisia tabaci genn. Mungbean yellow mosaic disease caused by mungbean yellow mosaic begomovirus mymv is the most destructive viral disease of mungbean and mashbean not only in pakistan but also in india, bangladesh, srilanka and adjacent area of southeast asia bakar, 1981. Comparisons of the sequence homology among the four viruses could be useful in. Studies on host resistance of mung bean against mung bean yellow mosaic virus in the agroecological condition of lateritic zone of west bengal. Very severe yellow discoloration of leaves covering 90.
Yellow mosaic disease of mungbean is the most serious disease and the main constraint in increasing the production of this crop. The mung bean vigna radiata, alternatively known as the green gram, maash persian. Note the green and yellow mottling of the leaf surface mungbean yellow mosaic virus what is mungbean yellow mosaic virus. Management of mungbean yellow mosaic virus mymv in mungbean. Wilczek, also known as greengram, green bean, mash bean, golden gram and green soy is an important source of dietary protein across asia. Causes a yellow mosaic disease of mung bean vigna radiata starting as. Mungbean yellow mosaic virus fs plant health australia. Yellow mosaic disease of cultivated legumes in southeast asia, is caused by mungbean yellow mosaic india virus mymiv and mungbean yellow mosaic virus mymv belonging to the genus begomovirus of the family geminiviridae. Bean yellow mosaic virus is a plant pathogenic virus in the genus potyvirus and the virus family potyviridae. As in bcmv, bymv will have contrasting yellow or green mosaic markings on the foliage of the infected plant. Mungbean yellow mosaic virus mymv causes one of the most destructive diseases in mungbean.
Avmu1683 shiny and bold seeded, high yielding, resistant to mungbean yellow mosaic virus vigna strain and urdbean strain and mungbean yellow mosaic india virus. Symptoms of infection with mung bean yellow mosaic virus. Twenty four genotypes of mung bean vigna radiata l. The virus is spread between plants by the silverleaf white fly bemisia tabaci, which is present in australia. Materials and methods twelve mung bean genotypes namely nm92, nm2006, azri 2006, nm121, nm54, nm2021, 014358, 014287, 014530. Status of the mung bean yellow mosaic virus mymv disease. Effect of different pesticides on incidence of mungbean.
Mung bean yellow mosaic virus how is mung bean yellow. Studies on mung bean yellow mosaic virus disease on greengram. Mungbean yellow mosaic india virus mymiv belonging to the genus begomovirus causes the yellow mosaic disease in a number of economically important edible grain legumes including mungbean vigna radiata, urdbean vigna mungo and soybean glycine. Aug 19, 2019 pant v, gupta d, choudhury nr, malathi vg, varma a, mukherjee sk 2001 molecular characterization of the rep protein of the blackgram isolate of indian mung bean yellow mosaic virus. Phylogenetic analysis of the cp gene sequence of the virus with other begomoviruses showed cluster next to french bean yellow mosaic virus fbymv gkvk isolate and lima bean yellow mosaic virus by. One hundred genotypeslines of mungbean germplasm were screened against mymv during summer season under field conditions at narc, islamabad. An infector row technique was used for evaluating parents, f1, f2 and f3 plants for mymv resistance.
Mung bean, like any other leguminous plant, is susceptible to diseases caused by fungi, bacteria and viruses. Among the three, the viruses are the most crucial group of plant pathogens, which. Evaluation of mung bean germplasm for resistance against mung. Pdf molecular markerassisted genotyping of mungbean. Mungbean yellow mosaic virus mymv is a plant pathogenic virus of the family geminiviridae. Pcna interacts with indian mung bean yellow mosaic virus. Wilczek with mungbean yellow mosaic virus mymv was investigated in a replicated, largeplot trial in 2002.